Table 1.
Parallel mutations are commonly observed across experimental conditions.
| Species | Target | Product/Function | Mut | P value | Frequency across replicates | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| cstBA mono | cstBA co | cstCi mono | cstCi co | Flu mono | Flu co | |||||
| A. johnsonii | oatA | O-acetyltransferase OatA | 15 | 1 × 10−40 | 1 | 1 | 0.75 | – | 1 | 0 (0/2) |
| P. putida | gacS | Sensor protein GacS | 9 | 5 × 10−24 | 0.25 | 0 | 0.75 | 0.5 | 0.25 | 0.5 |
| P. putida | flagelluma | Flagellar components | 7 | 3 × 10−17 | 0.25 | 0 | 0.25 | 0.25 | 0.75 | 0.25 |
| P. putida | fleQ | Transcriptional regulator FleQ/cyclic-di-GMP | 5 | 3 × 10−15 | 0.25 | 0.25 | 0 | 0.25 | 0.25 | 0.25 |
“Target” refers to the most commonly mutated gene or function; “Mut” refers to the number of mutations observed across lines. “P value” testing whether the number of mutations in a gene is random (Poisson cumulative expectation). “Frequency across replicates” in different conditions (cstBA constant Benzyl Alcohol, cstCi constant Citrate, Flu fluctuating environment, mono monoculture, co co-culture). Frequencies reflect how replicable are mutations across replicates: 1 = mutation in 4 out of 4 replicates; 0.75 = 3 out of 4; 0.5 = 2 out of 4; 0.25 = 1 out of 4; 0 = 0 out of 4.
aflagellum: genes related motility (GO: 2000147); mutations in flgH (5), fliQ (1), flhB (1). P value shown was calculated for flgH.