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. 2021 Apr;191(4):720–729. doi: 10.1016/j.ajpath.2021.01.003

Figure 4.

Figure 4

Systemic treatment with recombinant pigment epithelium–derived factor (rPEDF) maintains regulatory T-cell (Treg) phenotype in dry eye disease (DED). To delineate the effect of PEDF on dysfunctional Tregs in DED, mice were treated with i.p. injection of rPEDF (1 μg/mL) or murine serum albumin (MSA; control) once daily for 7 days. A: Schematic diagram showing the time points of DED induction, i.p. rPEDF or MSA injections, and tissue harvesting. B: Representative flow cytometry plots showing the frequency of CD4+CD25+FOXP3+ Tregs. C: Bar charts showing the frequency of forkhead box P3 (FOXP3), in mice treated with rPEDF or MSA. D: Bar charts showing the expression levels of FOXP3 in mice treated with rPEDF or MSA. Data are presented as a representative experiment of the four performed, each consisting of three mice per group. Data are presented as means ± SEM (C and D). ∗∗P < 0.01. DLN, draining lymph node; MFI, mean fluorescence intensity; qd, once a day.