Table 1.
Gas chromatography and headspace sampler parameters for the analysis of ethanol in blood.
| Parameter | Value | |
|---|---|---|
| Inlet temperature | 110°C | |
| Injection mode | Split | |
| Pressure | 85 kPa | |
| Column flow | 2.78 mL/min | |
| Linear velocity | 42.30 cm/s | |
| Purge flow | 3.00 mL/min | |
| Split ratio | 5.00 | |
| Oven temperature | 40°C isothermal | |
| Oven temperature (headspace sampler) | 60°C | |
| Syringe temperature | 70°C | |
| Fill volume | 1.75 mL | |
| Oscillation time | 0.50 minutes on | 0.10 minutes off |
| Sample speed | 5.0 mL/min | |
| Injection speed | 80 mL/min | |
| Sample speed | 5.0 mL/min | |
The method was designed specifically for quantitation of ethanol in blood samples. This method was validated prior to the study initialisation.