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. Author manuscript; available in PMC: 2022 Mar 9.
Published in final edited form as: Anal Chem. 2021 Feb 22;93(9):4217–4222. doi: 10.1021/acs.analchem.0c04764

Figure 1. Diagram showing comparison between traditional workflow and MOST workflow.

Figure 1.

For traditional proteomics, a 60 min nLC-MS/MS was applied, in which gradient was from water to acetonitrile/water (80:20,v/v). For traditional lipidomics, a 30 min HPLC-MS/MS was applied, in which gradient was from acetonitrile/water (70:30,v/v). to isopropanol/acetonitrile (90:10,v/v). For MOST, a special 90 min HPLC-MS/MS was applied, in which gradient was from water to isopropanol/acetonitrile (90:10,v/v). Mobile phase additives were detailed in Experiment Section.