Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 23;60(9):678–688. doi: 10.1021/acs.biochem.1c00003

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 The Authors. Published by American Chemical Society

Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Aβ42 fibrillation kinetics in the presence of proSP-C BRICHOS T187R. (A) Individual fits (colored lines) of normalized aggregation traces of 3 μM Aβ42 alone (black) and in the presence of 0.1, 0.3, 0.5, 1, and 1.5 molar equivalents of proSP-C BRICHOS T187R (light blue to dark blue empty circles) using a kinetic nucleation model in which the combined rate constants and are free fitting parameters. (B) Seeded reaction profiles of 3 μM Aβ42 with 0.6 μM preformed fibrils (black) in the presence of 0.1, 0.3, 0.5, 0.7, and 1.0 molar equivalent of proSP-C BRICHOS T187R (light pink to dark purple). Data represent the average of four replicates. The black rectangle shows a close-up of the onset of aggregation. Colored lines are corresponding fits to the data using a linear regression. (C) Effects of relative rate constants k_nk₊ (green), k₊ (pink), and k₊k₂ (blue) from the fits shown in panels A and B. Rates have been normalized to Aβ42 in the absence of BRICHOS. Data represent the mean ± the standard deviation of four or five replicates.

Inline graphic — Aβ42 fibrillation kinetics in the presence of proSP-C BRICHOS T187R. (A) Individual fits (colored lines) of normalized aggregation traces of 3 μM Aβ42 alone (black) and in the presence of 0.1, 0.3, 0.5, 1, and 1.5 molar equivalents of proSP-C BRICHOS T187R (light blue to dark blue empty circles) using a kinetic nucleation model in which the combined rate constants and are free fitting parameters. (B) Seeded reaction profiles of 3 μM Aβ42 with 0.6 μM preformed fibrils (black) in the presence of 0.1, 0.3, 0.5, 0.7, and 1.0 molar equivalent of proSP-C BRICHOS T187R (light pink to dark purple). Data represent the average of four replicates. The black rectangle shows a close-up of the onset of aggregation. Colored lines are corresponding fits to the data using a linear regression. (C) Effects of relative rate constants k_nk₊ (green), k₊ (pink), and k₊k₂ (blue) from the fits shown in panels A and B. Rates have been normalized to Aβ42 in the absence of BRICHOS. Data represent the mean ± the standard deviation of four or five replicates.