Figure 1.

Neuropathological analysis of the white matter cervical spinal cord of 6‐week‐old wild‐type and Plp1 overexpressing (PLP‐tg^66/66) mice. Seven hundred‐nanometer semithin epoxy embedded cervical spinal cord sections from WT (A) and PLP‐tg^66/66 (B) mice stained with toluidine blue. Photographs taken at 100× length of ventral column white matter (A, B). Seventy‐nanometre ultrathin sections stained with uranyl acetate and lead citrate from WT (C, E) and PLP‐tg^66/66 (D, F) obtained from the same block than semithin sections. Electron micrographs of sections from WT (C) and PLP‐tg^66/66 (D) magnification ×25 000 and from WT (E) and PLP‐tg^66/66 (F) magnification ×50 000. Large unmyelinated axons were very frequently observed in PLP‐tg^66/66 mice (B, D). Of note, when myelin was preserved, the sheets exhibited normal compaction and periodicity, but reduced thickness (Black filled arrows; D and F). An increase in the density or size of the mitochondria was identified in PLP‐tg^66/66 oligodendrocytes, with some of the mitochondria appearing degenerated (Black empty arrows; D). Quantifications made in 3 WT and 3 PLP‐tg^66/66 confirmed the increase in size of both axon and oligodendrocyte cytoplasm in PLP‐tg^66/66 as well as the increase in mitochondria size and number. Statistical analysis was performed by two‐way ANOVA; *P < 0.05, **P < 0.01; ***P < 0.001.