Table 1.
Identification of carbonylated proteins by peptide mass fingerprint and/or TOF‐TOF PSD. Twenty‐one proteins related to energetic/mitochondrial/glutamate metabolism were identified.
| Identified protein | Access/short name | pI | Mw (Da) | MASCOT Score |
|---|---|---|---|---|
| Glycolysis | ||||
| Gamma‐enolase | ENOG | 4.99 | 47609 | 213 |
| Pyruvate kinase isozymes M1/M2 | KPYM | 7.18 | 58378 | 107 |
| Alpha‐enolase | ENOA | 6.37 | 47453 | 193 |
| Phosphoglycerate kinase 1 | PGK1 | 8.02 | 44921 | 124 |
| Fructose‐bisphosphate aldolase A | ALDOA | 8.31 | 39787 | 211 |
| Glyceraldehyde‐3‐phosphate dehydrogenase | G3P, GAPDH | 8.44 | 36072 | 151 |
| Fructose‐bisphosphate aldolase C | ALDOC | 6.67 | 39769 | 229 |
| Phosphoglycerate mutase 1 | PGAM1 | 6.67 | 28928 | 109 |
| Triosephosphate isomerase | TPIS | 6.9 | 27038 | 288 |
| Citric acid cycle | ||||
| Aconitase hydratase, mitochondrial | ACO2 | 7.87 | 85372 | 62 |
| Malate dehydrogenase, mitochondrial | MDHM | 8.93 | 36045 | 142 |
| Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial | IDH3A | 6.27 | 40069 | 185 |
| Dihydrolipoyl dehydrogenase, mitochondrial | DLDH | 7.99 | 54751 | 120 |
| Electron transport chain | ||||
| NADH‐ubiquinone oxidoreductase 75 kDa subunit, mitochondrial | NDUS1, CxI sub‐75kD | 5.51 | 80724 | 199 |
| ATP synthase subunit alpha, mitochondrial | ATPA, CxV subα | 9.22 | 59830 | 138 |
| ATP synthase subunit beta, mitochondrial | ATPB, CxV subβ | 5.19 | 56265 | 167 |
| Glutamine metabolism | ||||
| Glutamate dehydrogenase 1, mitochondrial | DHE3 | 8.05 | 61640 | 252 |
| Glutamine synthetase | GLNA | 6.64 | 42834 | 149 |
| Aspartate aminotransferase, mitochondrial | AATM, GOT | 9.13 | 47780 | 103 |
| Other | ||||
| Creatine kinase B‐type | KCRB | 5.4 | 42971 | 164 |
| Voltage‐dependent anion‐selective channel protein 1 | VDAC1 | 8.55 | 32502 | 208 |