Figure 1.

Tissue characterization and TMA methodology. Panel A; Luxol Fast Blue (LFB) staining and antibodies to MOG and HLA were used to characterize whole sections from FFPE MS tissue blocks in terms of demyelination and inflammation. All sampled white matter lesions were classified as chronic active inflammatory lesions and cortical gray matter lesions classified based on their location within the neocortex. Three cores per region of interest, each of 0.6 mm diameter (0.28 mm² area) were extracted using a MicroArrayer from the donor block and transferred to a recipient in a predetermined grid location. Panel B; examples of whole TMAs stained with LFB, MOG and HLA and cores from each area of interest; normal appearing white matter (NAWM), white matter lesion edge and lesion center, normal appearing gray matter (NAGM), gray matter lesion edge and lesion center. Cores from lesion edge and lesion center were combined and labeled white matter lesion (WML) and gray matter lesion (GML) for analysis. Scale bars are shown. Abbreviations: LFB; Luxol fast blue, MOG; myelin oligodendrocyte glycoprotein, FFPE; formalin fixed paraffin embedded.