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. 2017 Jul 30;28(4):507–520. doi: 10.1111/bpa.12546

Figure 4.

Figure 4

Complement regulator expression in MS white matter. Each of panels A–G: Box plots show total immunostained cell counts and examples of typical staining in WML (top) and NAWM (bottom) of C pathway regulators; C1INH, CR1, FH (as stained with polyclonal anti‐FH antibody, and mAb Ox‐24 and MBI‐15), the terminal complex regulator, clusterin and FH‐Related protein (FHR). All regulators are shown to be increased in WML compared with NAWM (all P < 0.05). Panel A: WML and NAWM IHC from case MS230, inset shows C1INH astrocytic staining from case MS203. Panel B: CR1 positive glia in WML of case MS273 and reduced staining in NAWM of case MS230. Panel C: Positive FH glial staining in an active NAWM peri‐plaque region of case MS197 and lack of cellular staining in NAWM of case MS106. Panel D: Terminal pathway regulator, clusterin positive glia of case MS197 WML edge and reduced in NAWM of case MS230. Monoclonal antibodies Ox‐24 and MBI‐15 both recognize epitopes on FH (N‐terminal and mid‐section, respectively), MBC‐125 is specific for FHR proteins 1/2/5. Panel E: Ox‐24 positive glia from WML and NAWM of case MS377. Panel F: ramified MBI‐15 glia of WML center from case MS377 and reduced staining in NAWM of case MS273. Panel G: FHR positive active glia from WML edge of case MS197 and lack of cellular staining in NAWM of the same case. Median, IQR and full range of data shown. Abbreviations: NAGM; normal appearing gray matter, NAWM; normal appearing white matter, C1INH; C1 inhibitor, CR1; complement receptor 1, FH; Factor H; FHR; Factor H related proteins.