Table 1.
Primary antibodies used.
| Antibody | Type | IHC/IF dilution | WB dilution | Source |
|---|---|---|---|---|
| α‐Fodrin | Monoclonal | n/a | 1:1000 | Enzo Life Sciences, Inc. |
| β‐actin | Monoclonal | n/a | 1:5000 | Sigma‐Aldrich, Inc. |
| μ‐Calpain | Polyclonal | n/a | 1:1000 | Sigma‐Aldrich, Inc. |
| APH1 | Polyclonal | 1:200 | 1:1000 | Kind gift from Dr. G. Thinakaran, The University of Chicago, US |
| APP (clone Y188) | Monoclonal | n/a | 1:5000 | Abcam Inc. |
| APP (clone 369) | Polyclonal | n/a | 1:1000 | Kind gift from Dr. G. Thinakaran, The University of Chicago, US |
| APP (clone CTM1) | Polyclonal | 1:2000 | n/a | Kind gift from Dr. G. Thinakaran, The University of Chicago, US |
| BACE1 | Polyclonal | 1:200 | 1:1000 | EMD Millipore Co. |
| CD68 (clone ED1) | Monoclonal | 1:50 | n/a | AbD Serotec |
| Cleaved Caspase‐3 | Monoclonal | 1:200 | n/a | Cell Signaling Technology |
| GFAP | Polyclonal and monoclonal | 1:500 | n/a | Dako |
| Nicastrin (N‐19) | Polyclonal | 1:200 | 1:500 | Santa Cruz Biotechnology, Inc. |
| p44/42 MAPK (ERK1/2) | Monoclonal | n/a | 1:1000 | Cell Signaling Technology, Inc. |
| Phospho‐p44/42 MAPK (ERK1/2) | Polyclonal | n/a | 1:1000 | Cell Signaling Technology, Inc. |
| Phospho‐CREB (Ser133) | Monoclonal | n/a | 1:1000 | Cell Signaling Technology, Inc. |
| PEN2 | Polyclonal | 1:200 | 1:1000 | Kind gift from Dr. G. Thinakaran, The University of Chicago, US |
| PS1 | Polyclonal | 1:500 | 1:3000 | Kind gift from Dr. G. Thinakaran, The University of Chicago, US |
| sAPPα (2B3) | Monoclonal | n/a | 1:1000 | IBL Co., Ltd |
| sAPPβ (Wild‐type) | Monoclonal | n/a | 1:1000 | IBL Co., Ltd |
Abbreviations: IHC/IF = Immunohistochemistry/Immunofluorescence; WB = western blotting; n/a = not used in specified application.