Fig. 2.

Coverage and variant calling between intra-cDNA and inter-cDNA replicates. (A) Sequencing coverage of the 98 amplicons of ARTIC V3 panel from four representative replicates of sample S5. Green bars represent the amplicons generated using the ARTIC original primer set, and orange bars represent the amplicons generated using the alternative V3 primers. Red arrows point at representative amplicons missing in only one replicate. (B) Integrative Genomics Viewer (IGV) visualization of four representative sequencing replicates of sample S5 in the region 19,080–19,180 of the SARS-Cov-19 genome. Black arrows indicate variants called only in one replicate. The amplicon was not amplified in replicate S5 2.1. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)