Fig. 3.

Merging sequencing replicates can improve coverage and genotypability. (A) Mean percentage genome coverage (± standard deviations). (B) Mean percentage genotypability (± standard deviations). Both genome coverage and genotypability were calculated for single replicates or after merging all possible combinations of two or six replicates, starting from the same total sequencing reads (****p < 0.0001, Mann Whitney U test). (C) The coverage fraction contributed by each of the six replicates generated from sample S5. (D) Percentage of genome coverage after merging different numbers of replicates from sample S5, and from three other COVID-19-positive swab samples, namely samples 3270 (E), 4572 (F), 4173 (E), whose sequencing results are reported in Table S12.