Fig. 4.

Comparison of SARS-CoV-2 sequencing and mapping results obtained using the KAPA and Illumina library preparation kits. (A) Distribution of the number of fragments generated using the KAPA Hyper Prep and Illumina DNA Prep kits for the same set of 30 replicates. (B) Visualization of mean sequencing coverage on a representative ARTIC amplicon using the KAPA and Illumina library kits. Given the overlap with adjacent amplicons, the 5′ and 3′ ends show increased coverage. (C) Mean coverage (± standard deviations) and (D) mean genotypability (± standard deviations) of sequencing libraries prepared from the 30 replicates using either the KAPA or Illumina kits. The 100PE results were obtained from the 150PE dataset by in silico trimming.