Table 4.
Tm values of predicted siRNA (guide strand and passenger strand).
| RNA oligo sequences 21 nt guide (5′→3′) 21 nt passenger (5′→3′) |
Seed-duplex stability (Tm)oC |
|
|---|---|---|
| Guide | Passenger | |
| UCAAUAGUCUGAACAACUGGU CAGUUGUUCAGACUAUUGAAG |
11.6 °C | 17.8 °C |
| UACCUUUUUAGCUUCUUCCAC GGAAGAAGCUAAAAAGGUAAA |
17.3 °C | 19.1 °C |
| UGUUUAGCAAGAUUGUGUCCG GACACAAUCUUGCUAAACACU |
20.9 °C | 19.3 °C |
| UUAAAACACCCUCUUGAACAA GUUCAAGAGGGUGUUUUAACU |
7.2 °C | 20.4 °C |
| UAGUAGAUACACAAACACCAG GGUGUUUGUGUAUCUACUAGU |
18.9 °C | 19.3 °C |
| UGUUAAGUACCCAUCUACCAC GGUAGAUGGGUACUUAACAAU |
12.9 °C | 20.3 °C |
| UGUAACUGGACACAUUGAGCC CUCAAUGUGUCCAGUUACACA |
19.0 °C | 20.5 °C |
| UUCAUGUUGGUAGUUAGAGAA CUCUAACUACCAACAUGAAGA |
20.5 °C | 18.9 °C |
| UCUCUAUCAGACAUUAUGCAA GCAUAAUGUCUGAUAGAGACC |
20.2 °C | 5.6 °C |
| AUAGAUGUCAACUCAAAGCCA GCUUUGAGUUGACAUCUAUGA |
20.3 °C | 16.6 °C |