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. 2018 Dec 21;29(2):232–244. doi: 10.1111/bpa.12679

Figure 1.

Figure 1

Design of screening strategy and quality controls. A. Screening workflow comprises reformatting library, positive controls (light grey) and negative controls (dark grey) into 384‐well plates, reverse transfecting cells, incubating plates for 72 h, removing culture media, lysing content, dispensing fluorophore‐coupled antibodies and performing a TR‐FRET readout. B. Histograms displaying positive and negative control signal occurrences (22 each per plate across 24 plates) for U‐251 MG (Z′: 0.453), CHP‐212 (Z′: −0.403) and SH‐SY5Y M4 (Z′: 0.072) mimic screens with illustrations of each respectively expressed transcript.