Figure 2.

miRNA mimic screening identified 19 candidate hits. A. Dual‐flashlight plots displaying SSMD vs. Log₂ fold change (FC) with illustrations of each respectively expressed PRNP/Prnp transcript for U‐251 MG (black), CHP‐212 (blue) and SH‐SY5Y M4 (red) screens. Sample distribution angles illustrated in plots indicate cell‐line dependent variability in PrP^C expression, with U‐251 MG exhibiting stronger sample replicability, exemplified by high SSMDs, and CHP‐212 exhibiting larger biological effect sizes, as illustrated by greater Log₂ fold changes. B. SSMD overlaps between U‐251 MG (SSMD ≥5, ≤−5) and CHP‐212 (≥3, ≤−3) (left plot) identified 12 candidates (blue crosses). SSMD overlaps between U‐251 MG (≥3, ≤−3) and M4 (≥3, ≤−3) (right plot) identified 7 candidates (red crosses). A majority of candidates displayed consistent miRNA‐induced down‐ or upregulation in overlapped screens, whereas three hits exhibited divergent PrP^C regulation. (Bottom right quadrant of left plot: 1 blue cross. Top left quadrant of right plot: 1 blue and 1 red cross). C. SSMD overlaps between CHP‐212 (≥3, ≤−3) and SH‐SY5Y M4 (≥3, ≤−3) shows comparative distribution of mimic candidates.