Figure 4.

Reporter assay confirms predicted PRNP 3′UTR sites as targets for majority of hits. A. Co‐transfection of mimics with wt PRNP 3′UTR or respective mutation harboring reporters found that 12 out of 14 TargetScan7.2 predictions were functional sites for miRNAs. Only miR‐376b‐3p did not significantly regulate Firefly luciferase via the 3′UTR and only miR‐5588‐3p could not be confirmed to elicit its effect through its prospective target site. B. Assessment of miR‐124‐3p, 192‐3p and 299‐5p with wt PRNP 3′UTR or CDS reporters found that none of these miRNAs significantly regulated Firefly luciferase through either site.