Table 2.
Primer list used for qRT‐PCR.
| Genebank account number | Name | Primers | Target |
|---|---|---|---|
| NM_000660 | TGFB1 |
5′‐TACCTGAACCCGTGTTGCTC‐3′ 5′‐GTATCGCCAGGAATTGTTGC‐3′ |
Intron‐spanning (exons 2–3) |
| NM_003238 | TGFB2 |
5′‐CAATGCCAACTTCTGTGCTG‐3′ 5′‐ATATAAGCTCAGGACCCTGCTG‐3′ |
Intron‐spanning (exon 6–7) |
| NM_005901 | SMAD2 |
5′‐GTTTTGAAGCCGTCTATCAGC‐3′ 5′‐TTGTTACCGTCTGCCTTCG‐3′ |
Intron‐spanning (exon 10–11) |
| NM_005902 | SMAD3 |
5′‐GAAGATGGAGAAACCAGTGACC‐3′ 5′‐ATTCGGGGATAGGTTTGGAG‐3′ |
Intron‐spanning (exons 4–5) |
| NM_005359 | SMAD4 |
5′‐TGGAGCTCATCCTAGTAAATGTG‐3′ 5′‐AGGAAATCCTTTCCGACCAG‐3′ |
Intron‐spanning (exon 2–3) |
| NM_005904 | SMAD7 |
5′‐AGGGGGAACGAATTATCTGG‐3′ 5′‐TCGTCTTCTCCTCCCAGTATG‐3′ |
Intron‐spanning (exon 3–4) |
| NM_001130916 | TGFBR1 |
5′‐CGTGCTGACATCTATGCAATG‐3′ 5′‐TCAACTGATGGGTCAGAAGG‐3′ |
Intron‐spanning (exon 7–8) |
| NM_001024847 | TGFBR2 |
5′‐CTGTGTCGAAAGCATGAAGG‐3′ 5′‐AGTCAACGTCTCACACACCATC‐3′ |
Intron‐spanning (exon 6–7) |
| NM_000602 | PAI‐1 |
5′‐CAACTTGCTTGGGAAAGGAG‐3′ 5′‐CGTCTGATTTGTGGAAGAGG‐3′ |
Intron‐spanning (exons 3–4) |
| NM_212482 | FN1 |
5′‐GCAGTGGCTGAAGACACAAG 5′‐CCTGCCATTGTAGGTGAATG |
Intron‐spanning (exon 7–8) |
| NM_000088 | Col1A1 |
5′‐ ATGACGTGATCTGTGACGAGAC 5′‐TTCTTGGTCGGTGGGTGAC |
Intron‐spanning (exons 2–3) |
| NM_000090 | Col3A1 |
5′‐GACCTGAAATTCTGCCATCC 5′‐GCATGTTTCCCCAGTTTCC |
Intron‐spanning (exons 48–49) |
| NM_003194 | TBP |
5′‐CGCCGAATATAATCCCAAGC‐3′ 5′‐GAAAATCAGTGCCGTGGTTC‐3′ |
Intron‐spanning _ reference gene |
| NM_000983 | RPL22 |
5′‐TCGCTCACCTCCCTTTCTAA‐3′ 5′‐TCACGGTGATCTTGCTCTTG‐3′ |
Intron‐spanning _ reference gene |
| NM_000190 | HMBS |
5′‐GCAACGGCGGAAGAAAA‐3′ 5′‐CGAGGCTTTCAATGTTGCC‐3′ |
Intron‐spanning _ reference gene |