Figure 13.

α‐Synuclein fibril injections in the OB/AON elicit sex and age‐dependent behavioral deficits. Mice were infused in the right olfactory bulb/anterior olfactory nucleus with either preformed α‐synuclein fibrils (5 μg/1 μL) or an equivalent volume of PBS (1 μL). The buried pellet test for olfactory capacity was conducted at 3 months post‐infusion in all groups, and again at 10 months post‐infusion in only the oldest group of mice (the only survivors at that time). A blinded observer calculated the latency to approach an exposed peanut (A) and the latency to approach a buried peanut (B). The cylinder test was performed at 4.5 months post‐infusion (C‐D). Shown are the number of rears (C) and forelimb contacts (D) with the walls of the cylinder in 10 minutes. The novel object/place recognition tests were performed at 5.5 months post‐infusion (E‐H). Note that the oldest group of mice were not subjected to any behavior assay after 6 months post‐infusion, other than the buried pellet test. Two‐way ANOVAs were followed by the Bonferroni post hoc correction. Shown are the mean and SD from an n of 3–16 mice per group (see Methods and Figure 1 for animal numbers). *P ≤ 0.05, ***P ≤ 0.001 PBS vs. fibrils; +P ≤ 0.05, ++P ≤ 0.01 vs. 3‐month male group. ~P ≤ 0.05 vs. 11‐month male group. Abbreviations are defined in Table S2. To view the original, higher resolution Adobe Illustrator or EPS files, please link to https://dsc.duq.edu/pharmacology/ or https://www.dropbox.com/sh/a6r5ylg1tco6trm/AAC9Mb2gWuP29ABbmdGuNoaJa?dl=0