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. 2021 Apr 7;20:80. doi: 10.1186/s12934-021-01571-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Hydrolysis of starchy substrates for various times by NFAmy13B. Eight mg/mL potato, corn, and wheat starch were hydrolyzed by NFAmy13B for 2 to 20 h in the presence of 1.25 mM CaCl₂, and the products were hydrolyzed by reducing ends (a) and HPAEC-PAD (b). M1, glucose; M2, maltose; M3, maltotriose; M4, maltotetraose. Note: Means with different small letters are significantly different (p < 0.05). Each experiment was performed in triplicate