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. 2021 Apr 7;21:181. doi: 10.1186/s12903-021-01543-x

Table 1.

Primers used in qPCR

Forward primer Reverse primer Amplicon size (bp)
Total bacteria 5′-TCCTACGGGAGGCAGCAGT -3′ 5′-GGACTACCAGGGTATCTAATCCTGTT-3′ 466
S. mutans 5′-GCCTACAGCTCAGAGATGCTATTCT-3′ 5′-GCCATACACCACTCATGAATTGA-3′ 98
Lactobacillus spp. 5′-TGGAAACAGGTGCTAATACCG-3′ 5′-GTCCATTGTGGAAGATTCCC-3′ 231
S. Sobrinus 5′-TTCAAAGCCAAGACCAAGCTAGT-3′ 5′-CCAGCCTGAGATTCAGCTTGT-3′ 88
ldh gene 5′-CTTGATACTGCTCGTTTCCGTC-3′ 5′-GAGTCACCATGTTCACCCAT-3′ 93
F-ATPase gene 5′-CGGATGCGTGTTGCTCTTACTG-3′ 5-GGCTGATAACCAACGGCTGATG-3′ 167