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. 2021 Apr 8;22:89. doi: 10.1186/s13059-021-02302-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Integrative analysis of planarian trypsin- and ACME-dissociated datasets. a Analysis workflow. b UMAP visualization of 21,610 trypsin-dissociated S. mediterranea cells reanalyzed from Plass et al., colored by cluster identity and annotated on the basis of marker genes. c UMAP visualization of 19,025 ACME-dissociated S. mediterranea cells colored by transfer label analysis from trypsin-dissociated cell analysis (a). The numbers in the plot denote the center of the cells transferred with each of the trypsin-dissociated cell clusters. d UMAP visualization of 40,635 cells integrating trypsin-dissociated and ACME-dissociated cells. Clusters are colored by transfer label analysis from ACME-dissociated cell analysis