Figure 5.

Lack of effects of ethanol on astrocyte viability. Astrocyte viability in highly confluent cultures was assessed using MTT assays after (A) chronic ethanol exposure and after (B) recovery. No significant differences were observed in any of the conditions tested. Lactate dehydrogenase activity (LDH) was also used as a measure of cytotoxicity, with leakage of LDH into the media an indicator of compromised membrane integrity. Ethanol exposure reduced media LDH concentrations, and had no effect during recovery (numbers in parentheses indicate the n for each group). Striped bars indicate control groups. Solid black bars indicate ethanol‐exposed groups. *P < 0.05.