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. 2016 Aug 28;27(4):449–458. doi: 10.1111/bpa.12421

Figure 4.

Figure 4

Expression of components of the mTOR signaling cascade in the rat brain following UCL/HI at P6 and during normal development. Representative Western blot analysis of (A) mTOR downstream targets phospho‐p70S6K & p70S6K and (B) phospho‐S6 and S6 at five different developmental time points in UCL/HI animals (HI) and age and sex matched normoxic controls (N). (C) Western blots quantification of phospho‐S6 (pS6) expression during the same gestational period (n = 8). Histograms represent averaged optical density normalized to actin, and expressed relative to the mean controls values (n = 8). *P < 0.05 vs. normoxic controls.