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. 2014 Apr 29;24(5):475–493. doi: 10.1111/bpa.12134

Figure 6.

figure

Characterization of aquaporin 4 ( AQP 4) in the astrocytic compartment. Immediately around the cavitation cores, AQP4 (red) is present especially in the inner rim of glial cells [stained for glial fibrillary acidic protein (GFAP) green] (arrows in series A). Dotted line in series A delimitates the cavitation core of the infarct. AQP4 signal surrounds the arms of an astrocyte's cytoskeleton (B). An inset from image B is enlarged in image C, and after z‐scanning and deconvolution, three‐dimensional projections show that AQP4 does not colocalize with GFAP, but rather surrounds the tortuosities of this astrocytic process on its entire length (D). No vessel was present around this cell supporting the idea that AQP4 is not only expressed in astrocytic end feet. Not all GFAP‐positive astrocytes were also positive for AQP4, the arrow depicts a GFAP+/AQP4− astrocyte and the arrow head illustrates a GFAP+/AQP4+ astrocyte (E). Not all AQP4+ astrocytes were GFAP+, the arrow illustrating a GFAP−/AQP4+ astrocyte, and the arrow head a GFAP+/AQP4− astrocyte (F). Perilesional gemistocytes also express AQP4, but restricted mostly to their processes (G). Scale bars represent 50 μm unless denominated otherwise.