Table 2.
The antibodies utilized in this study
| Name | Clonality | Staining pattern | Immunization target | Dilution | Source |
|---|---|---|---|---|---|
| Anti‐AchR | Goat, polyclonal | Neuronal membrane | A synthetic peptide KRPGEDKVRPACQHKQ from an internal region of human nicotinic acetylcholine receptor alpha 7 | 1:100 | NBP1‐52375, Novus Biologicals, Cambridge, UK |
| Anti‐aquaporin 4 | Rabbit, polyclonal | Aquaporin 4 on astrocyte membrane | Amino acids 244–323, C‐terminal, of human aquaporin 4 | 1:1000 | sc‐20812, Santa Cruz, Heidelberg, Germany |
| Anti‐collagen type IV | Mouse, IgG1k, clone CIV22 | Basement membranes | Purified pepsin fragments of type IV collagen isolated from human kidney | 1:100 | M0785, Dako, Glostrup, Denmark |
| Anti‐collagen type IV | Rabbit, polyclonal | Basement membranes | Collagen type IV from human and bovine placenta | 1:5000, with tyramide | NB120‐6586, Novus Biologicals |
| Anti‐CD31 | Mouse, IgG1k, clone JC70A | Vascular endothelium | Cell membrane preparation from the spleen of a patient with hairy cell leukemia | 1:100 | M0823, Dako |
| Anti‐GFAP | Mouse, IgG1k, clone GA5 | Astrocyte cytoskeleton | Glial fibrillary acidic protein from porcine spinal cord | 1:500 | ABIN125137, Antibodies online, Aachen, Germany |
| Anti‐GFAP | Rabbit, polyclonal | Astrocyte cytoskeleton | GFAP isolated from cow spinal cord | 1:20 000 | Z0334, Dako |
| Anti‐GLT‐1 (EAAT2) | Mouse, IgG2a, clone 1H8 | GLT‐1 molecule on astrocyte membrane | Recombinant protein corresponding to the C‐terminal domain of human glutamate transporter 1 protein | 1:10 | LS‐C87838, Lifespan Biosciences, Seattle, WA, USA |
Antigen retrieval utilized: microwaving in 0.1 M Citrate pH = 6.
AchR = acetylcholine receptor; EAAT = excitatory amino acid transporter; GFAP = glial fibrillary acidic protein; GLT‐1 = glutamate transporter‐1; IgG = immunoglobulin G.