Table 2.
Morphometrically determined area of extracellular matrix molecule deposition in the thoracic spinal cord of mock‐ and TMEV‐infected mice. Abbreviations: PSR = picrosirius red stain; Mock = mock‐infected mice; TMEV = Theiler's murine encephalomyelitis virus‐infected mice; dpi = days post infection.
| Histochemical stain or immunohistochemically detected molecule | Positive area per total white matter area [%] | |||||
|---|---|---|---|---|---|---|
| 0 dpi | 7 dpi | |||||
| Mock | TMEV | P‐value | Mock | TMEV | P‐value | |
| Azan | 0.032 (0.010;0.091)† | 0.010 (0.003;0.015) | 0.082 | 0.013 (0.005;0.034) | 0.013 (0.006;0.022) | 1.000 |
| PSR, blue color | 0.000 (0.000;0.002) | 0.013 (0.000;0.099) | 0.082 | 0.002 (0.000;0.017) | 0.017 (0.005;0.026) | 0.167 |
| PSR, red color | 0.025 (0.000;0.084) | 0.000 (0.000;0.021) | 0.126 | 0.011 (0.020;0.019) | 0.003 (0.000;0.009) | 0.167 |
| Aggrecan | 0.004 (0.000;0.011) | 0.007 (0.000;0.122) | 0.931 | 0.017 (0.000;0.026) | 0.023 (0.007;0.066) | 0.548 |
| Brevican | 0.009 (0.000;0.033) | 0.008 (0.000;0.021) | 1.000 | 0.014 (0.005;0.023) | 0.011 (0.010;0.014) | 0.381 |
| Collagen I | 0.191 (0.046;0.589) | 0.100 (0.025;1.430) | 0.792 | 0.175 (0.102;0.252) | 0.060 (0.053;1.474) | 0.548 |
| Collagen IV | 1.990 (0.736;3.282) | 1.710 (1.307;4.073) | 0.762 | 1.249 (0.559;2.890) | 1.154 (0.691;1.132) | 0.714 |
| Decorin | 0.167 (0.097;0.527) | 0.145 (0.041;0.585) | 0.792 | 0.236 (0.102;0.407) | 0.127 (0.055;0.312) | 0.381 |
| Entactin | 2.484 (1.236;3.101) | 2.212 (1.537;2.628) | 0.699 | 2.460 (1.699;3.718) | 2.030 (1.552;3.213) | 0.413 |
| Fibronectin | 0.023 (0.013;0.054) | 0.078 (0.000;0.146) | 0.662 | 0.085 (0.000;0.216) | 0.028 (0.022;0.068) | 0.381 |
| Laminin | 0.053 (0.019;0.177) | 0.054 (0.009;0.119) | 1.000 | 0.149 (0.031;0.208) | 0.152 (0.011;0.169) | 0.548 |
| Neurocan | 0.102 (0.031;0.270) | 0.188 (0.103;0.297) | 0.082 | 0.066 (0.018;0.157) | 0.126 (0.101;0.155) | 0.262 |
| Perlecan | 0.422 (0.205;0.881) | 0.537 (0.229;1.115) | 0.485 | 0.577 (0.225;0.751) | 0.390 (0.214;0.778) | 0.686 |
| Phosphacan | 99.972 (99.869;99.992) | 99.884 (99.814;99.994) | 0.429 | 99.962 (99.834;99.966) | 99.953 (99.862;99.984) | 1.000 |
| Tenascin‐C | 0.366 (0.170;0.463) | 0.336 (0.091;0.551) | 1.000 | 0.374 (0.211;0.471) | 0.320 (0.054;0.488) | 0.905 |
| Histochemical stain or immunohistochemically detected molecule | Positive area per total white matter area [%] | |||||
|---|---|---|---|---|---|---|
| 14 dpi | 28 dpi | |||||
| Mock | TMEV | P‐value | Mock | TMEV | P‐value | |
| Azan | 0.004 (0.003;0.032) | 0.015 (0.000;0.038) | 0.662 | 0.015 (0.004;0.024) | 0.040 (0.000;0.093) | 0.429 |
| PSR, blue color | 0.008 (0.000;0.045) | 0.006 (0.003;0.011) | 0.548 | 0.011 (0.000;0.082) | 0.463 (0.003;0.656) | 0.052 |
| PSR, red color | 0.013 (0.000;0.062) | 0.000 (0.000;0.017) | 0.222 | 0.033 (0.004;0.050) | 0.016 (0.000;0.094) | 0.662 |
| Aggrecan | 0.021 (0.000;0.057) | 0.036 (0.000;0.089) | 0.662 | 0.013 (0.000;0.034) | 0.005 (0.000;0.025) | 0.537 |
| Brevican | 0.008 (0.000;0.023) | 0.000 (0.000;0.004) | 0.171 | 0.009 (0.000;0.029) | 0.005 (0.000;0.015) | 0.429 |
| Collagen I | 0.510 (0.071;0.894) | 0.289 (0.022;6.405) | 0.841 | 0.162 (0.096;0.337) | 0.201 (0.176;0.266) | 0.171 |
| Collagen IV | 1.147 (0.278;2.634) | 2.976 (1.398;5.525) | 0.114 | 1.127 (0.263;2.308) | 3.485 (2.010;5.245) | 0.009* |
| Decorin | 0.363 (0.032;0.466) | 0.393 (0.071;0.487) | 0.931 | 0.094 (0.034;0.109) | 0.188 (0.040;0.219) | 0.247 |
| Entactin | 2.684 (1.897;3.338) | 2.531 (1.408;3.422) | 0.818 | 2.905 (1.564;3.444) | 2.471 (1.294;2.913) | 0.180 |
| Fibronectin | 0.032 (0.003;0.110) | 0.026 (0.000;0.175) | 1.000 | 0.447 (0.000;0.088) | 0.027 (0.008;0.244) | 0.792 |
| Laminin | 0.050 (0.017;0.200) | 0.111 (0.006;0.288) | 0.690 | 0.044 (0.016;0.124) | 0.169 (0.006;0.543) | 0.329 |
| Neurocan | 0.091 (0.017;0.175) | 0.059 (0.033;0.122) | 0.662 | 0.119 (0.062;0.153) | 0.085 (0.058;0.159) | 0.792 |
| Perlecan | 0.390 (0.103;0.685) | 0.286 (0.081;0.975) | 0.931 | 0.439 (0.216;0.786) | 0.461 (0.224;2.411) | 0.699 |
| Phosphacan | 99.960 (99.921;99.996) | 99.982 (99.865;99.998) | 1.000 | 99.891 (99.670;99.977) | 99.540 (99.191;99.971) | 0.177 |
| Tenascin‐C | 0.211 (0.112;0.670) | 0.279 (0.195;0.618) | 0.476 | 0.353 (0.231;0.466) | 1.709 (0.032;4.392) | 0.257 |
| Histochemical stain or immunohistochemically detected molecule | Positive area per total white matter area [%] | |||||
|---|---|---|---|---|---|---|
| 56 dpi | 98 dpi | |||||
| Mock | TMEV | P‐value | Mock | TMEV | P‐value | |
| Azan | 0.018 (0.003;0.024) | 0.090 (0.009;0.420) | 0.030* | 0.010 (0.003;0.030) | 0.474 (0.003;3.550) | 0.083 |
| PSR, blue color | 0.003 (0.000;0.005) | 0.030 (0.000;1.049) | 0.247 | 0.004 (0.000;0.013) | 0.207 (0.000;0.493) | 0.257 |
| PSR, red color | 0.010 (0.002;0.044) | 0.101 (0.000;1.362) | 0.177 | 0.014 (0.000;0.027) | 0.497 (0.021;1.312) | 0.019* |
| Aggrecan | 0.002 (0.000;0.005) | 0.006 (0.000;0.039) | 0.352 | 0.002 (0.000;0.031) | 0.003 (0.000;0.042) | 0.931 |
| Brevican | 0.021 (0.000;0.031) | 0.004 (0.000;0.015) | 0.171 | 0.011 (0.000;0.032) | 0.013 (0.000;0.038) | 0.792 |
| Collagen I | 0.098 (0.050;0.815) | 0.354 (0.013;0.637) | 0.730 | 0.036 (0.011;0.064) | 1.953 (0.138;13.755) | 0.010* |
| Collagen IV | 0.857 (0.043;2.085) | 3.196 (2.073;7.421) | 0.009* | 0.509 (0.090;1.708) | 8.710 (3.539;15.868) | 0.010* |
| Decorin | 0.080 (0.039;0.100) | 0.073 (0.030;0.118) | 0.762 | 0.041 (0.009;0.183) | 0.212 (0.046;5.173) | 0.052 |
| Entactin | 2.438 (2.063;3.173) | 2.475 (1.699;5.222) | 0.699 | 2.356 (0.922;2.874) | 3.149 (1.986;5.711) | 0.052 |
| Fibronectin | 0.010 (0.003;0.048) | 0.005 (0.000;0.089) | 0.792 | 0.018 (0.000;0.035) | 0.430 (0.045;2.388) | 0.004* |
| Laminin | 0.019 (0.013;0.085) | 0.046 (0.007;1.248) | 0.352 | 0.059 (0.005;0.198) | 1.189 (0.089;11.777) | 0.017* |
| Neurocan | 0.053 (0.025;0.109) | 0.075 (0.036;0.150) | 0.662 | 0.051 (0.017;0.243) | 0.146 (0.059;0.436) | 0.329 |
| Perlecan | 0.369 (0.105;0.853) | 0.627 (0.369;5.084) | 0.240 | 0.304 (0.021;0.539) | 4.409 (0.307;5.262) | 0.017* |
| Phosphacan | 99.928 (99.878;99.989) | 99.071 (88.127;99.982) | 0.126 | 99.888 (99.854;99.980) | 91.831 (89.665;96.469) | 0.004* |
| Tenascin‐C | 0.350 (0.074;0.538) | 1.680 (0.299;4.033) | 0.026* | 0.215 (0.208;0.619) | 1.887 (0.247;3.707) | 0.032* |
| Histochemical stain or immunohistochemically detected molecule | Positive area per total white matter area [%] | ||
|---|---|---|---|
| 196 dpi | |||
| Mock | TMEV | P‐value | |
| Azan | 0.012 (0.004;0.022) | 1.474 (0.011;2.714) | 0.030* |
| PSR, blue color | 0.016 (0.000;0.022) | 0.033 (0.004;0.885) | 0.310 |
| PSR, red color | 0.005 (0.000;0.021) | 2.561 (0.030;3.792) | 0.008* |
| Aggrecan | 0.008 (0.002;0.022) | 0.012 (0.000;0.022) | 0.610 |
| Brevican | 0.013 (0.000;0.035) | 0.006 (0.003;0.012) | 0.352 |
| Collagen I | 0.015 (0.000;0.051) | 1.447 (0.108;7.642) | 0.010* |
| Collagen IV | 1.009 (0.133;1.638) | 7.458 (1.495;8.584) | 0.038* |
| Decorin | 0.086 (0.020;0.356) | 1.152 (0.020;7.004) | 0.167 |
| Entactin | 2.515 (1.745;2.879) | 5.202 (3.046;8.751) | 0.004* |
| Fibronectin | 0.002 (0.000;0.034) | 0.393 (0.007;6.377) | 0.038* |
| Laminin | 0.085 (0.026;0.140) | 3.050 (0.094;10.859) | 0.017* |
| Neurocan | 0.072 (0.048;0.088) | 0.156 (0.057;1.194) | 0.310 |
| Perlecan | 0.507 (0.143;0.870) | 5.018 (2.908;5.711) | 0.008* |
| Phosphacan | 99.968 (99.908;99.990) | 97.669 (87.386;99.877) | 0.029* |
| Tenascin‐C | 0.295 (0.076;0.453) | 1.693 (0.067;6.600) | 0.177 |
Data are given as percentage of positively labeled area per total white matter area.
*
Significant difference between TMEV‐infected and mock‐infected mice with P < 0.05 as detected by the Mann–Whitney U‐test.
†
Median (Minimum;Maximum).