Skip to main content
. 2012 Dec 27;23(4):402–412. doi: 10.1111/bpa.12013

Figure 3.

figure

Calsenilin affected Notch cleavage in HT‐22 cells subjected to oxygen glucose deprivation (OGD). A. The overexpression of calsenilin in standard conditions (left panels) increased Notch intracellular domain (NICD) (red) levels. Under OGD (right panels), calsenilin overexpression dramatically increased NICD (red) levels. Original magnification: 1000×. Scale bar: 50 μm. B. The overexpression of calsenilin potentiated OGD‐induced cell death. HT‐22 cells were transfected with either vector or calsenilin constructs. Calsenilin overexpression increased NICD, and the phosphorylation of p65 (p‐p65) in cultured cells. After 6 h of OGD, NICD and phospho‐p65 levels increased further. C. Quantification of NICD, p‐p65 and p65 levels normalized to β‐actin levels as a loading control. Values are the mean with standard error of measurement. *P < 0.05, ***P < 0.001 vs. vector, # P < 0.05 vs. vector + OGD 6 h.