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. 2013 Jan 9;23(4):413–425. doi: 10.1111/bpa.12014

Figure 7.

figure

PRDX3 is a direct target gene of miR‐383. (Top) A schematic diagram showing the predicted miR‐383 binding site on PRDX3 3'UTR. The nucleotide after the stop codon is defined as “1” on PRDX3 3'UTR. The potential miR‐383 binding site residues at nts 346–369 of PRDX3 3'UTR. A perfect complementary base pairing is observed at the seed region of miR‐383 and sequences on PRDX3 3'UTR. This miR‐383/PRDX3 3'UTR base pairing is conserved across six species, as indicated by the box. (Bottom) Luciferase reporter assay demonstrated that miR‐383 directly interacted with PRDX3 3'UTR. DAOY cells (left) and ONS‐76 cells (right) were co‐transfected with wild‐type PRDX3 3'UTR (pGL3‐PRDX3) or mutant PRDX3 3'UTR (pGL3‐MUT PRDX3) luciferase reporter plasmid as well as miR‐383 or negative control. Luciferase activity was measured at 48 h post‐transfection. Data represent the firefly luciferase activity standardized with Renilla luciferase activity.