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. 2011 Sep 16;22(2):159–174. doi: 10.1111/j.1750-3639.2011.00515.x

Figure 2.

Figure 2

Expression of MHC molecules and tumor antigens on glioblastoma multiforme (GBM) primary cell lines. A. GBM neurosphere (NS) and adherent (Adh) cells were stained with isotype controls (open histograms) or specific labeled antibodies against HLA‐ABC, HLA‐DR, IL13Rα2, EGFRvIII and EGFR (closed histograms), and then analyzed by flow cytometry. For EGFR expression, NS cell lines were grown for 48 h without (open histograms with thick lines) or with EGF (closed histograms). A representative primary cell line GBM#2 is shown (see Supporting Information Figure S4 for the others cell lines). B. The mean of specific fluorescence intensity of the protein expression obtained in each NS and Adh cell line was determined as described in Figure 1 (n = 8 and 5, respectively). The thick gray bar indicates the mean of specific fluorescence intensity of protein expression obtained in all cell lines. Cell lines were considered as positive for the expression of proteins of interest when the specific fluorescence intensity was more than 2 (dashed line). *P < 0.05. ns = not statistically different. C. Tumor antigen mRNA expression on GBM cell lines was analyzed by transcriptomic microarray experiments. Total mRNA from GBM cell lines was extracted and analyzed for a gene expression profile. Results are expressed as the mRNA expression fluorescence intensity.