Figure 3.

A. Western blot of subunits NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8, 19 kDa (NDUFB8), succinate dehydrogenase complex, subunit B, iron sulphur (lp) (SDHB), ubiquinol‐cytochrome c reductase core protein II (UQCRC2), cytochrome c oxidase subunit II (COX2) and ATP synthase, H+ transporting, mitochondrial F1 complex, and alpha subunit 1 (ATP5A) using OXPHOS antibody; ATP synthase, H+ transporting mitochondrial F1 complex, O subunit (ATP5O) and ubiquinol‐cytochrome c reductase binding protein (UQCRB) antibodies in mediodorsal thalamus in FFI and control cases. β‐actin and VDAC were used to normalize total protein and mitochondria protein loading, respectively. B. Densitometric analysis shows significant reduction of NDUFB8, SDHB, UQCRC2, and COX2 normalized with β‐actin and VDAC in FFI compared with controls. ATP5A and UQCRB protein levels were preserved. Results were analyzed by Graphpad Prism with Student's t‐test when the distribution was normal and with Mann–Whitney test if distribution was not normal as assessed with the Kolmogorov–Smirnov normality test. Differences are considered statistically significant at *P < 0.05, **P < 0.01, and ****P < 0.0001. ATP5O shows a significant reduction when normalized with β‐actin but only a trend with VDAC.