Skip to main content
. 2016 Mar 28;27(1):51–63. doi: 10.1111/bpa.12361

Figure 5.

Figure 5

The effect of NaHS on caspase‐3 activation after SAH. (A) Representative images show double immunofluorescent staining of active caspase‐3 and NeuN of prefrontal cortex (PFC). Scale bar = 50 μm. (B) The PFC extracts were subjected to Western blot analysis using an antibody against cleaved caspase‐3, total caspase‐3 were used to evaluate protein loading. (C) Bar graphs showing quantification of the protein levels of cleaved caspase‐3 and caspase‐3 were determined by Image‐Pro Plus 6·0. Results were expressed as cleaved caspase‐3/caspase‐3 ratio. n = 3. (D) Bar graphs showing quantification of active caspase‐3/NeuN‐positive cells. n = 4. Values represent the mean ± SD. ***p < 0.001 SAH vs. Sham; #p < 0.05, ##p < 0.01 SAH + NaHS vs. SAH. 1 Sham, 2 Sham + NaHS, 3 SAH, 4 SAH + NaHS.