Effect of NaHS on BDNF expression. (A) The relative expression levels of BDNF mRNA in the PFC were analyzed by semiquantitative RT‐PCR. Each value was normalized to β‐actin. Bar graphs showing quantification of mRNA levels of BDNF were determined by Image‐Pro Plus 6·0. n = 4. (B) The levels of BDNF in the PFC at 48 h after SAH were measured by western blotting, and β‐actin was used to evaluate protein loading. Bar graphs showing quantification of the protein levels of BDNF were determined by Image‐Pro Plus 6·0; n = 4. Values represent the mean ± SD, **p < 0.01, ***p < 0.001 SAH vs. Sham; #p < 0.05, ##p < 0.01, SAH + NaHS vs. SAH. 1 Sham, 2 Sham + NaHS, 3 SAH, 4 SAH + NaHS.