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. 2012 Aug 14;23(1):82–99. doi: 10.1111/j.1750-3639.2012.00617.x

Figure 6.

figure

Scatter plots with median illustrating the results of the real‐time quantitative polymerase chain reaction for the analysis of normalised mRNA levels per 100 ng transcribed RNA of the gelatinases MMP‐2 and MMP‐9 in naturally traumatized dogs (A,B) and organotypic spinal cord slice cultures (C,D). Group 1 = controls, group 2 = dogs with acute SCI, group 3 = dogs with subacute SCI. 0, 3, 9 = days in culture. Statistically significant differences are marked with an asterisk [Kruskal–Wallis test with subsequent pairwise Mann–Whitney U ‐test, alpha adjusted according to the method of Bonferroni (A,B); Friedman test and subsequent pairwise signed rank test (C,D)]. (A) MMP‐2 mRNA is transiently downregulated in dogs with acute SCI compared with controls. (B) MMP‐9 shows a significant upregulation in dogs with acute SCI compared with controls. (C) In organotypic spinal cord slices MMP‐2 shows a delayed upregulation on day 9 compared with day 3 in culture. (D) MMP‐9 is upregulated on day 3 and 9 in cultivated spinal cord slices compared with the control tissue of 0 days. MMP = matrix metalloproteinases; SCI = spinal cord injury.