. 2012 Aug 14;23(1):82–99. doi: 10.1111/j.1750-3639.2012.00617.x

Table 4.

Target gene, sequence, amplicon length and GenBank accession number of primers used for real–time quantitative polymerase chain reaction analysis.

Target gene	Primer sequence	Amplicon length (BP)	GenBank accession
MMP‐2	Forward: 5′‐GGAGATCTTCTTCTTCAAGGACCG‐3′	89	AF177217
MMP‐2	Reverse: 5′‐AGAATGTGGCTACCAGCAGGG‐3′	89	AF177217
MMP‐9	Forward: 5′‐CATGACATCTTCCAGTACCAAG‐3′	85	AB006421
	Reverse: 5′‐GGTTCACCTCATTCCGAGAA‐3′
	Probe: 5′‐FAMCTACTTCTGCCAGGACCGCTTCTACTTAMRA‐3′
HPRT	Forward: 5′‐GAGATGACCTCTCAACTTTAACTGAAAA‐3′	89	CFU16661
HPRT	Reverse: 5′‐GGGAAGCAAGGTTTGCATTG‐3′	89	CFU16661
EF‐1α	Forward: 5′‐CAAAAACGACCCACCAATGG‐3′	68	AY195837
EF‐1α	Reverse: 5′‐GGCCTGGATGGTTCAGGATA‐3′	68	AY195837
GAPDH	Forward: 5′‐GTCATCAACGGGAAGTCCATCTC‐3′	84	AB038240
GAPDH	Reverse: 5′‐AACATACTCAGCACCAGCATCAC‐3′	84	AB038240

BP = base pairs; EF‐1α = elongation factor 1α; GAPDH = glyceraldehyde‐3‐phosphate‐dehydrogenase; HPRT = hypoxanthine‐guanine phosphoribosyltransferase; MMP = matrix metalloproteinase.