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Serial reconstruction of an astrocytic plaque in CBD processed for double‐labeling immunofluorescence and confocal microscopy using antibodies to P‐tau (AT8, green) and GFAP (red). Note the localization of P‐tau in the distal regions of astrocytic branches and the irregular distribution of GFAP in the cytoplasm and astrocytic branches. Nuclei (blue) are stained with DRAQ5TM; bar = 35 μm.
