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. 2014 Jan 13;24(3):239–246. doi: 10.1111/bpa.12114

Figure 4.

figure

Three aberrations found only in epithelioid tumor cells (A–C) and one example of aberrations detected in all histological areas (D) by array CGH. A. A homozygous deletion in intron 3 of the LSAMP. The deletion was validated by differential PCR using two primer sets (fragments #1 and #2) located in the deleted part shown by array CGH. Numbers below each band of differential PCR indicate the ratio of amplification of the target compared with the CF reference gene. RT‐differential PCR showed LSAMP mRNA expression in diffusely infiltrating less atypical astrocytoma cells but not in epithelioid tumor cells. #1, 2 = fragments #1, 2. B. A heterozygous deletion in ODZ 3. The deletion was validated by FISH analysis. BAC clones used for the target (RP11‐713O21; 4q34.3–4q35.1) and reference (RP11–758 M15; 4p15.2) probes are indicated with red and green bars, respectively. C. A heterozygous deletion in LRP1B. D. A homozygous deletion in CDKN 2A/2B. Abbreviations: BAC = bacterial artificial chromosome; CGH = comparative genomic hybridization; DA = diffusely infiltrating less atypical astrocytoma cells; Ep = epithelioid tumor cells; FISH = fluorescence in situ hybridization; nc = normal control; PCR = polymerase chain reaction; RT = reverse transcription.