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. 2014 Nov 20;25(4):491–504. doi: 10.1111/bpa.12194

Figure 5.

figure

CD74 expression in vivo is restricted to microglia cells in normal central nervous system (CNS) tissues as well as glioma‐associated microglia/macrophages (GAMs) in gliomas. Representative double‐immunofluorescence (IF) stainings of CD74 (Alexa 488, green) and the microglia/macrophage marker Iba1 (Alexa 568, red) show a colocalization in (A) normal white (n = 4), (B) grey matter (n = 4) and (C) glioblastoma World Health Organization (WHO) grade IV (n = 15) samples (images A–C were taken with the C1 confocal microscope). No colocalization of the ligand migration inhibitory factor (MIF) (Alexa 488, green) and its receptor CD74 (Alexa 568, red) was seen in (D) normal brain or (E) glioblastoma WHO grade IV samples (images D–E were taken with the Eclipse 80i fluorescent microscope; original magnification A–D: 20×; E: 40×). In addition, pilocytic astrocytoma WHO grade I (n = 2) as well as WHO grade II (n = 2) and III (n = 2) astrocytomas were assessed revealing similar staining results (data not shown).