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. 2014 Nov 20;25(5):552–564. doi: 10.1111/bpa.12214

Figure 7.

figure

The spatial patterns of  p 62 and  NBR 1 in the mouse brain. A. Equal amounts of homogenates from the indicated regions were analyzed by immunoblotting (12 weeks of age, n = 2 in wild‐type mice). Antibodies against NBR1 (upper) or p62 (bottom) were used to detect endogenous proteins. NBR1 is mainly expressed in the olfactory bulb, temporal and occipital cortices, striatum, thalamus and hypothalamus. The highest expression of p62 is observed in the olfactory bulb, striatum, temporal and occipital cortices, hippocampus, thalamus, hypothalamus and medulla oblongata. β‐Actin is used as a loading control. B. Distribution patterns of NBR1 and p62 in the sagittal section of mice brains. NBR1 or p62 levels are normalized by β‐actin. The circles represent the size of the expression level. C. NBR1 immunostaining in Tg and Tg/KO mice (9 weeks of age, n = 6 per group). NBR1 immunoreactivity is mainly detected in neurons of the thalamus of both Tg and Tg/KO mice. Note the increased intensity of NBR1 immunoreactivity in Tg/KO mice compared with Tg mice. Bar = 30 μm.