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. Author manuscript; available in PMC: 2021 Apr 8.
Published in final edited form as: Cell Rep. 2020 Sep 8;32(10):108125. doi: 10.1016/j.celrep.2020.108125

Figure 2. Atf-6 Regulates ER Function and Lifespan through Its Conserved Target, Calreticulin.

Figure 2.

(A) Volcano plot of differentially expressed transcripts in atf-6(ok551) relative to N2.

(B) Relative transcript abundance of conserved UPR target genes in 1-day old atf-6 mutants (means ± SDs of RNA sequencing [RNA-seq] read counts normalized to wild-type, n = 4).

(C and D) qRT-PCR analysis of changes in UPR transcripts in wild-type worms between days 1 and 7 of adulthood (C), contrasted with changes in UPR transcripts in aging atf-6 mutants (D) (means ± 95% confidence intervals of 3 independent samples of ~100 worms; *p < 0.05, **p < 0.01, and ***p < 0.001).

(E) Lifespan analysis of atf-6(ok551) and crt-1(bz29) mutants (n = 100 animals per curve).