| Antibodies |
|
|
| phospho-AMPK (Thr172) |
Cell Signaling |
Cat# 2535; RRID:AB_331250 |
| Anti rabbit IgG, HRP linked Antibody |
Cell Signaling |
Cat# 7074S; RRID:AB_2099233 |
| Bacterial and Virus Strains |
|
|
|
E. coli OP50-1 |
CGC |
WBStrain00041971 |
|
E. coli HT115(DE3) |
Source Bioscience |
WBStrain00041079 |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| Thapsigargin |
Sigma Aldrich |
Cat# T9033 |
| Tunicamycin |
EMD Millipore |
Cat# 5.04570.0001 |
| Critical Commercial Assays |
|
|
| Taqman Universal Master Mix |
Thermo Fisher |
Cat# 4440040 |
| RNeasy Mini Kit |
QIAGEN |
Cat# 74104 |
| TruSeq Stranded mRNA kit |
Illumina |
Cat# 20020594 |
| Pierce ECL Western Blot substrate |
Thermo Fisher |
Cat# 32209 |
| Taqman crt-1 assay |
Thermo Fisher |
Ce02475941_g1 |
| Taqman ckb-2 assay |
Thermo Fisher |
Ce02445484_m1 |
| Taqman hsp-4 assay |
Thermo Fisher |
Ce02434878_g1 |
| Taqman xbp-1 s assay |
Thermo Fisher |
Ce04931896_g1 |
| Taqman xbp-1 assay |
Thermo Fisher |
Ce02421281_g1 |
| Taqman hsp-3 assay |
Thermo Fisher |
Ce02495956_g1 |
| Taqman pdi-6 assay |
Thermo Fisher |
Ce02496946_g1 |
| Deposited Data |
|
|
| Raw and analyzed RNA-Seq data |
This study |
GEO: GSE156037
|
| Experimental Models: Organisms/strains |
|
|
|
C. elegans strain: N2 Bristol |
CGC |
WB: N2 |
|
C. elegans strain: RB772: atf-6(ok551) |
CGC |
WB: RB772 |
|
C. elegans strain: SJ30: ire-1(zc14); zcIs4
|
CGC |
WB: SJ30 |
|
C. elegans strain: ZB1028: crt-1(bz29)
|
CGC |
WB: ZB1028 |
|
C. elegans strain:CZ19982: mcu-1(ju1154)
|
CGC |
WB: CZ19982 |
|
C. elegans strain: JT73: itr-1(sa73)
|
CGC |
WB: JT73 |
|
C. elegans strain: RB545: pek-1(ok275)
|
CGC |
WB: RB545 |
|
C. elegans strain: PS1631: itr-1(sy290); dpy-20(e1282)
|
CGC |
WB: PS1631 |
|
C. elegans strain: WBM926: wbmEx367[ges-1p::tomm20 (aa1-49)::GFP::unc54 3′UTR] |
Weir et al., 2017. |
N/A |
|
C. elegans strain: WBM947 atf-6(ok551); mcu-1(ju1154) |
This study |
N/A |
|
C. elegans strain: WBM1093: atf-6(ok551); xbIs1101[fln-1p::GCaMP3 + pRF4(rol-6)] II |
This study |
N/A |
|
C. elegans strain: WBM973: atf-6(ok551); itr-1(sa73) |
This study |
N/A |
|
C. elegans strain: WBM974: atf-6(ok551); itr-1(sy290) |
This study |
N/A |
|
C. elegans strain: WBM1046: atf-6(wbm27) |
This study |
N/A |
|
C. elegans strain: WBM1103: atf-6(ok551); wbmEx367 [ges-1p::tomm20 aa1-49::GFP::unc54 3′UTR)] |
This study |
N/A |
|
C. elegans strain: WBM1104: itr-1(sy290); wbmEx367 [ges-1p::tomm20 aa1-49::GFP::unc54 3′UTR] |
This study |
N/A |
|
C. elegans strain: WBM1134: itr-1(sa73); wbmEx367 [ges-1p::tomm20 aa1-49::GFP::unc54 3′UTR] |
This study |
N/A |
|
C. elegans strain: WBM1158: crt-1(bz29) V; atf-6(ok551) X |
This study |
N/A |
|
C. elegans strain: UN1108: xbIs1101[fln-1p::GCaMP3 + pRF4(rol-6)] |
Kovacevic et al., 2013 |
N/A |
|
C. elegans strain: WBM933: itr-1(sy290) |
This study |
N/A |
|
C. elegans strain: BUZ26: bugEx2[vha-6p::MLS::GCaMP7b::unc-54 UTR + vha-6p::MLS::mKate2::unc-54 UTR] |
This study |
N/A |
| Oligonucleotides |
|
|
| Primer: GCaMP7b F: CATCATGGTATGGCTAGCGTCGACTCATCACGTC |
This study |
N/A |
| Primer: GCaMP7b R: CGACCGGCGCTCAGTTGGAATTCTCACTTCGCTGTCATC |
This study |
N/A |
| Primer: mKate2 F CTAGC GTCTCCGAGCTCATTAAAG |
This study |
N/A |
| Primer: mKate2 R GAATTCTTAACGGTGTCCGAGCTTGG |
This study |
N/A |
| Primer: MLS F: TCGACTCTAGAGGATCCCCGGGATGTCCGTCCTGAC |
This study |
N/A |
| Primer: MLS R: GACGTGATGAGTCGACGCTAGCCATACCATGATG |
This study |
N/A |
| Recombinant DNA |
|
|
| vha-6p::MLS::GCaMP7b::unc-54 3′UTR |
This study |
N/A |
| vha-6p::MLS::mKate2::unc-54 3′UTR |
This study |
N/A |
| pGP-CMV-jGCaMP7b |
Dana et al., 2019 |
Addgene #104484 |
| Software and Algorithms |
|
|
| bcbioRNASeq |
Steinbaugh et al., 2018 |
http://bioinformatics.sph.harvard.edu/bcbioRNASeq |
