Figure 3.

Varicella zoster virus (VZV)–infected quiescent primary adult human perineurial cells (qHPNCs) contain Aβ42, amylin, and amyloid-like fibrillar structures. Mock- and VZV-infected qHPNCs were analyzed at 3 days postinfection by an immunofluorescence antibody assay using antibodies against VZV glycoprotein B (gB), Aβ42, and amylin, as well as by a thioflavin-T (Thio-T) fluorescence assay that detects β-sheets in amyloid-like fibrillar structures (prefibrillar oligomers and amyloid fibrils). A, Mock-infected qHPNCs did not contain VZV gB or Aβ42 and were Thio-T negative. VZV-infected qHPNCs contained VZV gB (red) and Aβ42 (yellow) and were Thio-T positive (green). B, Similarly, mock-infected qHPNCs did not contain VZV gB or amylin and were Thio-T negative. VZV-infected qHPNCs contained VZV gB (red) and amylin (yellow) and were Thio-T positive (green). Blue corresponds to DAPI staining of cell nuclei. Original magnification ×400.