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. 2020 Aug 18;223(7):1284–1294. doi: 10.1093/infdis/jiaa513

Figure 4.

Figure 4.

Herpes simplex virus type 1 (HSV-1)–infected quiescent primary adult human perineurial cells (qHPNCs) contain Aβ42 and amyloid-like fibrillar structures, but not amylin. Uninfected and HSV-1–infected qHPNCs were analyzed at 3 days postinfection by an immunofluorescence antibody assay using antibodies against HSV-1 ICP0, Aβ42, and amylin, as well as by a thioflavin-T (Thio-T) fluorescence assay that detects β-sheets in amyloid-like fibrillar structures (prefibrillar oligomers and amyloid fibrils). A, Uninfected qHPNCs did not contain HSV-1 ICP0 or Aβ42 and were Thio-T negative. HSV-1–infected qHPNCs contained HSV-1 ICP0 (red) and Aβ42 (yellow) and were Thio-T positive (green). B, Uninfected qHPNCs did not contain HSV-1 ICP0 or amylin and were Thio-T negative. HSV-1–infected qHPNCs contained HSV-1 ICP0 (red) and were Thio-T positive (green); unlike VZV-infected cells, HSV-1–infected cells did not contain amylin. Blue corresponds to DAPI staining of cell nuclei. Original magnification ×400.