Figure 6.

Global loss of Atf4 does not alter metabolic responses to dietary sulfur amino acid restriction (SAAR). (A) Overview of the genetic mouse models and experimental diets (top) and study outline (bottom) indicating the length of habituation (gray dashed line) and experimental (black solid line) periods in weeks as well as timing of interventions. Mice were placed in a comprehensive laboratory animal monitoring system (CLAMS) for 24 h during the fourth week of the experimental period. (B) Average cumulative food intake (grams per gram body weight) in male (left) and female (right) wild-type (WT) and Atf4 knockout (Atf4KO) mice over the 5-wk experimental period. (C) Average body weight change (g) in male (left) and female (right) mice over the 5-wk experimental period. (D) Average change in lean mass (percentage points) in male (left) and female (right) mice over the 5-wk experimental period. (E) Average change in fat mass (percentage points) in male (left) and female (right) mice over the 5-wk experimental period. (F) Average energy expenditure (kcal/h) measured in a CLAMS: males during dark photoperiod (left) and females during dark photoperiod (right). n = 3–11/group. *P < 0.05 with a main effect of diet. n.s. indicates no statistically significant differences at α = 0.05, as determined by two-factor ANOVA and ANCOVA using lean mass as covariate, in the case of energy expenditure. Bar chart values are presented as mean ± SEM.