Mechanical and structural properties of the bioink and the resulting scaffold. (a) Thermal gelation kinetics of the mixture of the bioink and sodium bicarbonate when the temperature is raised from 20 to 37°C. (b) Yield stress measurements for the gelatin-based PSIM (red), the pristine bioink (blue), and the bioink with the gelling agent (sodium bicarbonate, SC; green). (c) Storage moduli of the PVH vary with the pH. (d) Orthogonal control over viscoelasticity and stiffness with the PEG content. The stress relaxation timescale (τ1/2; Top) and the storage modulus (Bottom) as a function of the PEG content (%). NEH: Nanoporous elastic hydrogels made of glyoxal/glycol-chitosan. (e) Average pore sizes of NEH and PVHs with varying PEG content (%). Sample size, N=4. (f) Confocal images of NEH and PVHs containing rhodamine B-labeled chitosan. NEH is used as control and PVHs contain 0 or 2.8% PEG. (g) Frequency distributions of rhodamine B-labeled chitosan intensity. * represents p < 0.05, ** represents p < 0.01, *** represents p < 0.001, **** represents p < 0.0001, n.s. represents p ≥ 0.05.