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Babaodan (BBD) inhibits TLR1/2 and TLR3 dual stimulation-induced cytokine expression in Raw 264.7 macrophages. Raw 264.7 cells were grown on 12-well plates and incubated with different doses of BBD for 24 h, and this was followed by treatment with a combination of Poly(I:C) (10 μg/mL) and Pam3CSK4 (100 ng/mL) for 24 h. Supernatants were collected, and TNF-α and IL-6 concentrations were analyzed using the corresponding ELISA kits. *P < 0.05, **P < 0.01，***P < 0.001 vs. model group.
