Fig 1. Increased bacterial cell numbers in dual species biofilms is contact dependent with early C. albicans biofilm.

A two chamber Transwell assay was used to determine whether contact between early C. albicans biofilms was required for enhanced bacterial biofilm cell numbers. (A) The number of E. coli, (B) S. aureus and (C) MRSA cells in the lower chamber biofilm was quantified by qPCR. Micro-organisms in the two chambers were physically separated from each other but shared the same medium. (Mann Whitney statistical analysis; 3 independent experiments. ns: p > 0.05, *: p<0.05, error bars SD).