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. 2021 Apr 7;9(4):e002231. doi: 10.1136/jitc-2020-002231

Figure 2.

Figure 2

CD103+ cells in tumors are predominantly CD8+ TRM cells and are characterized by Ki-67 and immune checkpoint expression. (A) NSCLC patient tumors (n=6) analyzed by mass cytometry, with resulting data for total CD45+ cells (8000 downsampled cells per sample) aggregated and visualized by UMAP (left). Immune subset identities were determined by manual gating and projected onto the UMAP. Expression of CD103 across total CD45+ cells from NSCLC tumors overlaid onto the UMAP (right). (B) Frequency of the indicated immune subpopulations of total CD103+ cells for all nine tumors (n=6 NSCLC (solid dots), n=3 endometrial cancer (open dots)). (C) Expression pattern for markers of activation and dysfunction for NSCLC tumors in aggregate projected onto the UMAP of CD8+ T cells. (D) Comparison of expression frequency of indicated markers within CD8+ T cells between CD103+ (red) or CD103− (blue) subsets in either tumor (left) or adjacent tissue (right) across all tumor samples (n=9, solid dots for NSCLC, open dots for endometrial). Statistical analysis was conducted using two-way analysis of variance with Bonferroni correction for multiple comparisons. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. n.s., not significant; NSCLC, non-small cell lung cancer; PD-1, programmed cell death 1; TRM, tissue-resident memory T; UMAP, uniform manifold approximation and projection.