Figure 4.

3478 inhibited MV4-11 cells growth. MV4-11 cells were treated with JQ1 (A) or 3478 (B) for 48 h and analysed by CCK8 kits. The IC₅₀ values were calculated using GraphPad Prism 7.0. (C) The percentages of apoptotic cells after 48 h treatment (the lower-right quadrant of the FACS histograms (percentage of early apoptotic cells) and the upper-right quadrant (percentage of late apoptotic cells)) are shown. All results were representative images from three experiments. The MV4-11 cells were exposed to different concentrations of JQ1, 3478 or culture medium for 48 h (D) and 24 h (E), and stained with Annexin V-FITC and PI for apoptosis measurement by flow cytometry.