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. 2021 Apr 6;36(1):903–913. doi: 10.1080/14756366.2021.1906663

Figure 6.

Figure 6.

Compound 3478 downregulated the c-Myc both in MV4-11 cells and MV4-11 xenograft tumours through BRD4/c-Myc signalling pathway. (A) The protein expression of BRD4 and c-Myc after 3478 treatment for 24 h were measured by Western blotting. Quantification of protein level was normalised to GAPDH using densitometry. (B) The mRNA level of c-Myc in MV4-11 cells after 3478 intervention for 24 h was determined by real-time qPCR. GAPDH was used as the loading control. (C) The protein expression of BRD4 and c-Myc in MV4-11 xenograft tumours in the different groups were measured by Western blotting. Quantification of protein level was normalised to GAPDH using densitometry. (D) The mRNA level of c-Myc in MV4-11 xenograft tumours in the different groups were determined by RT-qPCR. GAPDH was used as the loading control. (E) Immunofluorescence analysis of c-Myc localisation and expression in MV4-11 transplanted tumour tissues in different groups (100×, scale bar represented 100 μm). Compared with control or model group: *p < 0.05, **p < 0.01, ***p < 0.001. Data in panels represented means ± SD from 10 animals. Independent experiments were performed in triplicate.